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The trick is to develop efficient and cost-effective cellulose degrading enzymes—cellulases—for converting cellulose into low-cost sugars. “Our longterm goal is to understand the molecular mechanisms of cooperative interaction between cellulases and how the morphological features of insoluble cellulose— such as pore size distribution and crystalline structure—influence the binding and catalytic activity of cellulase cocktails,” he says. In pursuing this research objective, Walker is collaborating with Professor Harold Craighead in Applied Engineering and Physics to use Total Internal Reflection Fluorescence Microscopy (TIRFM) and Fluorescence Correlation Spectroscopy (FCS) to observe and quantify the surface mobility of cellulases on microcrystalline cellulose. He also has an active collaboration with Professor James Gossett, director of the School of Civil and Environmental Engineering, on the pretreatment of switch grass, a perennial grass, to fractionate it into the various carbohydrate components and to yield a cellulose substrate that is more susceptible to subsequent enzymatic hydrolysis.
“The ready supply of methane on dairy farms makes farms one of the most likely stationary users of this fuel source,” Scott says, noting that methane biogas is just like natural gas except that it yields 60 percent of BTUs. Already, some farms capture methane from manure by trapping the gas and then they pump it into internal combustion generators. Fuel cells will be more efficient. They just have to become cheaper, Scott says. Can farms become energy sources for their surrounding communities and for related enterprises such as greenhouses and fish farms that have high energy demands? “That’s the question we’re asking,” Scott says, “which is part of an even bigger question: Can agriculture be a source not only for food but also for the raw materials for bio-industries and energy?” |